Regulatory

Part:BBa_K3095003:Design

Designed by: Jung Hun Park   Group: iGEM19_USP-Brazil   (2019-10-15)


Blue light regulated inducer circuit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2135
    Illegal NheI site found at 2158
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3988
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4786
    Illegal NgoMIV site found at 4858
    Illegal NgoMIV site found at 4948
    Illegal NgoMIV site found at 4966
    Illegal NgoMIV site found at 5458
    Illegal NgoMIV site found at 5751
    Illegal NgoMIV site found at 5845
    Illegal AgeI site found at 4500
    Illegal AgeI site found at 5626
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5515
    Illegal BsaI.rc site found at 4399


Design Notes

The circuit was reconstructed utilising the parts from the plasmids in Jesus & Voigt et al 2017, which was provided by the author.


Source

Based in Jesus & Voigt et al (https://doi.org/10.1038/nchembio.2390)

References